Abstract: Centella asiatica Linn. Commonly known as mandukparni or brahmi manduki is a traditional medical herb widely used as a brain tonic. Micro propagation offer rapid in vitro clonal multiplication of elite clones and further help in dissemination and ex situ conservation of this important yet endangered medicinal plant. In the present study, we have tried to optimize the invitro culture condition of centella asiatica for mass multiplication and callus culture study has also been initiated. Through our work, we have found that MS basal +1.0mg / l BAP+20gm/l sugar +8.0gm/l agar gives the best result for culture initiation as well as in auxiliary shoot proliferation. For rooting, MS+1.0mg/l NAA +200mg charcoal + 20 gm/l sugar +8 gm/l agar gave higher primary roots/ shoots with higher secondary roots.
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